A lab experiment on the bacteria staphylococcus epidermidis
Biological agent reference sheet (bars) agent: staphylococcus epidermidis bars – s epidermidis effective 23 october 2017 controlled document if viewed online uncontrolled if viewed in print. 27 lab 3 results i differential stains a gram stain draw and label examples of escherichia coli and staphylococcus epidermidis b acid-fast stain draw and label examples of mycobacterium smegmatis and staphylococcus epidermidis staphylococcus mycobacterium smegmatis & epidermidis questions: 1 what is the difference between a simple and a differential stain_____. I just got done doing a kirby bauer test on staphylococcus epidermidis, antibiotic used were gentamicin, penicillin novobiacin, 24 hours went by and they all measured 0mm so i am assuming this is resistance to all abx.
Lab report for staphylococcus aureus microbiology lab reportpractica #1btc307lamber amelingmeierthursday, september 18, 2008objectivesin this lab experiment two different types of bacteria, escherichia coli and staphylococcus aureus, were grown singly and mixed on four different types of agar in order to observe the varying morphologies within the colonies. The two types of bacteria used were staphylococcus epidermidis and bacillus subtilis the five mouthwashes we tested were scope, in doing this laboratory experiment, we wanted to see which mouthwashes were the most antibacterial ability of different mouthwashes. Capsule consists of polysaccharide or polypeptide-containing material which surrounds the cell wall of bacteria: primary stain in simple stain -mycobacterium - e coli is blue - mycobacterium smegmatis is red: primary stains in capsule stain organisms in gram stain experiment: staphylococcus epidermidis is gram-positive (purple. Staphlycoccus epidermidis was discovered in 1884 by friedrich julius rosenbach by noticing a difference between staphylococcus aureus and staphylococcus epidermidis (2) this bacteria is part of the human’s normal skin flora and naturally found on the body.
Start studying lab practical #1: gram staining, gram stains, gram-positive bacteria: staphylococcus epidermis (extra stuff) learn vocabulary, terms, and more with flashcards, games, and other study tools. S epidermidis is among the many bacteria that make up the normal skin flora this particular bacterium was first differentiated from other forms of staphylococcus in 1884 by friedrich julius rosenbach (1. Organism a was identified as staphylococcus epidermidis, due to the gram-positive cell wall, coccus shape, yellow colonies, positive catalase test, positive glucose + bromcresol purple slant, and negative mannitol salt agar plate, which are all characteristics of staphylococcus epidermidis. Smear a loopful of bacteria onto a clean microscope slide add a small drop of h 2 o 2 to the smeared organism with a dropper a positive test will begin to bubble within a few seconds.
Staphylococcus epidermidis is a gram-positive spherical bacterium that form irregular clusters staphylococci are commonly found on the skin and in the mucous membranes of humans and other mammals. Experiments in your lab manual -- do not write in your lab manual or notebook until after you staphylococcus epidermidis staphylococcus intermedius staphylococcus saprophyticus staphylococcus xylosus streptococcus bovis 12 don’t leave media open to the air – bacteria and fungi in the air can contaminate the media 13 don’t over. Staphylococcus epidermidis is a gram positive bacteria whose cell morphology resembles grape-like clusters of spheres it is a non-hemolytic organism that is catalase positive, nacl tolerant, and is unable to ferment mannitol (carson, 2015.
Microplate alamar blue assay for staphylococcus epidermidis biofilm susceptibility testing robin k pettit 1, 3, , and staphylococcus epidermidis is responsible for 50 to 70% of reported cases prior to each experiment, ab was brought to room temperature and vortexed exposure of ab to light was minimized throughout the experiments. Experiments, bacteria used, and results learn with flashcards, games, and more — for free 24 terms nsachovska microbiology lab final bact experiments, bacteria used, and results study play negative stain klebsiella pneumoniae=white rods on a purple background staphylococcus epidermidis=white balls in clusters on a purple. Staphylococcus epidermidis is often compared to staphylococcus aureus these bacteria are the two main pathogens in the genus due to the one million serious infections caused in hospitals per year s epidermidis is the dominant species that lives mostly on the skin while s aureus lives mostly on mucosal surfaces. This assignment must be completed prior to attending this lab fix a smear of staphylococcus epidermidis to the slide as follows: 1 b the capsule stain discussion many bacteria secrete a slimy, viscous covering called a capsule or glycocalyx this is usually composed of polysaccharide, polypeptide, or both. Unknown lab report unknown number 103 (staphylococcus aureus and klebsiella pneumoniae) michelle gudorp general microbiology spring 2013 introduction it is important to understand why a person would want to identify between different bacteria.
A lab experiment on the bacteria staphylococcus epidermidis
In the lab experiment today, we want to find the effects of uv irradiation on two species of bacteria ( serratia marcescens , a gram negative rod and staphylococcus aureus , a gram positive coccus) with and without different spf sunscreens. The purpose of the experiment of the use of differential, selective and enriched media will introduce “the use and function of specialized media for the selection and differentiation of microorganisms” (rudolph 89) using the following experiment and results will aid in isolating, charactering and identifying bacteria staphylococcus. Nb culture of staphylococcus epidermidis na plate of e coli dye tub dye reagents what names are given to the shapes seen in the 2 bacteria used in lab 5 why heat-fix the smear 6 why are basic dyes more useful than acidic dyes when staining bacteria 7 what is the disadvantage of having a really thick smear when staining.
Identification of unknown microbe through physiological and genetic characteristic culture bacteria previously impossible but for the sake of this experiment, the kinds of bacteria the genotype testing showed 9470% confidence level of certainty of the bacteria being staphylococcus epidermidis. Staphylococcus epidermidis growing on columbia cna agar 4 streak the same mixture for isolation (see fig 4 and fig 5 ) on a plate of macconkey agar (selective for gram-negative bacteria and differential for certain members of the bacterial family enterobacteriaceae . Table 1 shows the monochrome and gram staining of staphylococcus epidermidis and escherichia coli test monochrome staining gram staining bacteria cells staphylococcus epidermidis escherichia coli in this experiment, identification of bacteria was based on the physiological properties of the bacterial growth in liquid medium.
Staphylococcus epidermidis (s epi), which is known to be gram positive, and escherichia coli, which is known to be gram-negative if the gram stain procedure works as it should, s epi will be purple and e coli will be. In these experiments, the biofilm-positive strain s epidermidis rp62a, which produces the polysaccharide intercellular adhesin, was used in parallel, an s aureus clinical isolate which had been found to form biofilms on polystyrene surfaces was analyzed and served as a control. Unknown lab report for microbiology staphyloccocus epidermidis staphylococcus epidermidis is from the genusnstaphylococcus and the family staphylococ unknown lab report report by jessica unruh 12/06/12 microbiology after these tests it was determined that unknown bacteria b was staphylococcus epidermidis a last test was performed to.